Robust production of recombinant phosphoproteins using cell-free protein synthesis

Understanding the functional and structural consequences of site-specific protein phosphorylation has remained limited by our inability to produce phosphoproteins at high yields. Here we address this limitation by developing a cell-free protein synthesis (CFPS) platform that employs crude extracts f...

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Detalles Bibliográficos
Autores principales: Oza, Javin P., Aerni, Hans R., Pirman, Natasha L., Barber, Karl W., ter Haar, Charlotte M., Rogulina, Svetlana, Amrofell, Matthew B., Isaacs, Farren J., Rinehart, Jesse, Jewett, Michael C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4566161/
https://www.ncbi.nlm.nih.gov/pubmed/26350765
http://dx.doi.org/10.1038/ncomms9168

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4566161/
https://www.ncbi.nlm.nih.gov/pubmed/26350765
http://dx.doi.org/10.1038/ncomms9168

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