Deciphering Seed Sequence Based Off-Target Effects in a Large-Scale RNAi Reporter Screen for E-Cadherin Expression

Functional RNAi based screening is affected by large numbers of false positive and negative hits due to prevalent sequence based off-target effects. We performed a druggable genome targeting siRNA screen intended to identify novel regulators of E-cadherin (CDH1) expression, a known key player in epi...

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Detalles Bibliográficos
Autores principales: Adams, Robert, Nicke, Barbara, Pohlenz, Hans-Dieter, Sohler, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567318/
https://www.ncbi.nlm.nih.gov/pubmed/26361354
http://dx.doi.org/10.1371/journal.pone.0137640

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567318/
https://www.ncbi.nlm.nih.gov/pubmed/26361354
http://dx.doi.org/10.1371/journal.pone.0137640

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