Cargando…

AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach

Assembly cloning is increasingly replacing conventional restriction enzyme and DNA-ligase-dependent cloning methods for reasons of efficiency and performance. Here, we describe AQUA (advanced quick assembly), a simple and versatile seamless assembly cloning approach. We demonstrate the applicability...

Descripción completa

Detalles Bibliográficos
Autores principales: Beyer, Hannes M., Gonschorek, Patrick, Samodelov, Sophia L., Meier, Matthias, Weber, Wilfried, Zurbriggen, Matias D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567319/
https://www.ncbi.nlm.nih.gov/pubmed/26360249
http://dx.doi.org/10.1371/journal.pone.0137652