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Resolution and contrast enhancement of subtractive second harmonic generation microscopy with a circularly polarized vortex beam

We extend the subtractive imaging method to label-free second harmonic generation (SHG) microscopy to enhance the spatial resolution and contrast. This method is based on the intensity difference between two images obtained with circularly polarized Gaussian and doughnut-shaped beams, respectively....

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Detalles Bibliográficos
Autores principales: Tian, Nian, Fu, Ling, Gu, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568467/
https://www.ncbi.nlm.nih.gov/pubmed/26364733
http://dx.doi.org/10.1038/srep13580