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Resolution and contrast enhancement of subtractive second harmonic generation microscopy with a circularly polarized vortex beam
We extend the subtractive imaging method to label-free second harmonic generation (SHG) microscopy to enhance the spatial resolution and contrast. This method is based on the intensity difference between two images obtained with circularly polarized Gaussian and doughnut-shaped beams, respectively....
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568467/ https://www.ncbi.nlm.nih.gov/pubmed/26364733 http://dx.doi.org/10.1038/srep13580 |