Resolution and contrast enhancement of subtractive second harmonic generation microscopy with a circularly polarized vortex beam

We extend the subtractive imaging method to label-free second harmonic generation (SHG) microscopy to enhance the spatial resolution and contrast. This method is based on the intensity difference between two images obtained with circularly polarized Gaussian and doughnut-shaped beams, respectively....

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Detalles Bibliográficos
Autores principales: Tian, Nian, Fu, Ling, Gu, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568467/
https://www.ncbi.nlm.nih.gov/pubmed/26364733
http://dx.doi.org/10.1038/srep13580
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author Tian, Nian
Fu, Ling
Gu, Min
author_facet Tian, Nian
Fu, Ling
Gu, Min
author_sort Tian, Nian
collection PubMed
description We extend the subtractive imaging method to label-free second harmonic generation (SHG) microscopy to enhance the spatial resolution and contrast. This method is based on the intensity difference between two images obtained with circularly polarized Gaussian and doughnut-shaped beams, respectively. By characterizing the intensity and polarization distributions of the two focused beams, we verify the feasibility of the subtractive imaging method in polarization dependent SHG microscopy. The resolution and contrast enhancement in different biological samples is demonstrated. This work will open a new avenue for the applications of SHG microscopy in biomedical research.
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spelling pubmed-45684672015-09-23 Resolution and contrast enhancement of subtractive second harmonic generation microscopy with a circularly polarized vortex beam Tian, Nian Fu, Ling Gu, Min Sci Rep Article We extend the subtractive imaging method to label-free second harmonic generation (SHG) microscopy to enhance the spatial resolution and contrast. This method is based on the intensity difference between two images obtained with circularly polarized Gaussian and doughnut-shaped beams, respectively. By characterizing the intensity and polarization distributions of the two focused beams, we verify the feasibility of the subtractive imaging method in polarization dependent SHG microscopy. The resolution and contrast enhancement in different biological samples is demonstrated. This work will open a new avenue for the applications of SHG microscopy in biomedical research. Nature Publishing Group 2015-09-14 /pmc/articles/PMC4568467/ /pubmed/26364733 http://dx.doi.org/10.1038/srep13580 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Tian, Nian
Fu, Ling
Gu, Min
Resolution and contrast enhancement of subtractive second harmonic generation microscopy with a circularly polarized vortex beam
title Resolution and contrast enhancement of subtractive second harmonic generation microscopy with a circularly polarized vortex beam
title_full Resolution and contrast enhancement of subtractive second harmonic generation microscopy with a circularly polarized vortex beam
title_fullStr Resolution and contrast enhancement of subtractive second harmonic generation microscopy with a circularly polarized vortex beam
title_full_unstemmed Resolution and contrast enhancement of subtractive second harmonic generation microscopy with a circularly polarized vortex beam
title_short Resolution and contrast enhancement of subtractive second harmonic generation microscopy with a circularly polarized vortex beam
title_sort resolution and contrast enhancement of subtractive second harmonic generation microscopy with a circularly polarized vortex beam
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568467/
https://www.ncbi.nlm.nih.gov/pubmed/26364733
http://dx.doi.org/10.1038/srep13580
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AT gumin resolutionandcontrastenhancementofsubtractivesecondharmonicgenerationmicroscopywithacircularlypolarizedvortexbeam

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