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Reference Materials for Calibration of Analytical Biases in Quantification of DNA Methylation

Most contemporary methods for the quantification of DNA methylation employ bisulfite conversion and PCR amplification. However, many reports have indicated that bisulfite-mediated PCR methodologies can result in inaccurate measurements of DNA methylation owing to amplification biases. To calibrate a...

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Detalles Bibliográficos
Autores principales: Yu, Hannah, Hahn, Yoonsoo, Yang, Inchul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4569303/
https://www.ncbi.nlm.nih.gov/pubmed/26368560
http://dx.doi.org/10.1371/journal.pone.0137006