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Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters

The cloning of long DNA segments, especially those containing large gene clusters, is of particular importance to synthetic and chemical biology efforts for engineering organisms. While cloning has been a defining tool in molecular biology, the cloning of long genome segments has been challenging. H...

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Detalles Bibliográficos
Autores principales: Jiang, Wenjun, Zhao, Xuejin, Gabrieli, Tslil, Lou, Chunbo, Ebenstein, Yuval, Zhu, Ting F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4569707/
https://www.ncbi.nlm.nih.gov/pubmed/26323354
http://dx.doi.org/10.1038/ncomms9101