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Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters
The cloning of long DNA segments, especially those containing large gene clusters, is of particular importance to synthetic and chemical biology efforts for engineering organisms. While cloning has been a defining tool in molecular biology, the cloning of long genome segments has been challenging. H...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Pub. Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4569707/ https://www.ncbi.nlm.nih.gov/pubmed/26323354 http://dx.doi.org/10.1038/ncomms9101 |