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Micelle PCR reduces chimera formation in 16S rRNA profiling of complex microbial DNA mixtures

16S rRNA gene profiling has revolutionized the field of microbial ecology. Many researchers in various fields have embraced this technology to investigate bacterial compositions of samples derived from many different ecosystems. However, it is important to acknowledge the current limitations and dra...

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Detalles Bibliográficos
Autores principales: Boers, Stefan A., Hays, John P., Jansen, Ruud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4570986/
https://www.ncbi.nlm.nih.gov/pubmed/26373611
http://dx.doi.org/10.1038/srep14181