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Chloramphenicol Selection of IS10 Transposition in the cat Promoter Region of Widely Used Cloning Vectors
The widely used pSU8 family of cloning vectors is based on a p15A replicon and a chloramphenicol acetyltransferase (cat) gene conferring chloramphenicol resistance. We frequently observed an increase in the size of plasmids derived from these vectors. Analysis of the bigger molecular species shows t...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574204/ https://www.ncbi.nlm.nih.gov/pubmed/26375469 http://dx.doi.org/10.1371/journal.pone.0138615 |