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Chloramphenicol Selection of IS10 Transposition in the cat Promoter Region of Widely Used Cloning Vectors

The widely used pSU8 family of cloning vectors is based on a p15A replicon and a chloramphenicol acetyltransferase (cat) gene conferring chloramphenicol resistance. We frequently observed an increase in the size of plasmids derived from these vectors. Analysis of the bigger molecular species shows t...

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Detalles Bibliográficos
Autores principales: González-Prieto, Coral, Agúndez, Leticia, Llosa, Matxalen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574204/
https://www.ncbi.nlm.nih.gov/pubmed/26375469
http://dx.doi.org/10.1371/journal.pone.0138615