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RESOLFT Nanoscopy of Fixed Cells Using a Z-Domain Based Fusion Protein for Labelling

RESOLFT super-resolution microscopy allows subdiffraction resolution imaging of living cells using low intensities of light. It relies on the light-driven switching of reversible switchable fluorescent proteins (RSFPs). So far, RESOLFT imaging was restricted to living cells, because chemical fixatio...

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Detalles Bibliográficos
Autores principales: Ilgen, Peter, Grotjohann, Tim, Jans, Daniel C., Kilisch, Markus, Hell, Stefan W., Jakobs, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574475/
https://www.ncbi.nlm.nih.gov/pubmed/26375606
http://dx.doi.org/10.1371/journal.pone.0136233