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eIF4AII is dispensable for miRNA-mediated gene silencing

MicroRNAs (miRNAs) are short noncoding RNAs that regulate gene expression through partial complementary base-pairing to the 3′ untranslated region (UTR) of target mRNAs. Inhibition of translation initiation has been identified as an early event of miRNA-mediated gene repression, but the underlying m...

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Detalles Bibliográficos
Autores principales: Galicia-Vázquez, Gabriela, Chu, Jennifer, Pelletier, Jerry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574758/
https://www.ncbi.nlm.nih.gov/pubmed/26286746
http://dx.doi.org/10.1261/rna.052225.115
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author Galicia-Vázquez, Gabriela
Chu, Jennifer
Pelletier, Jerry
author_facet Galicia-Vázquez, Gabriela
Chu, Jennifer
Pelletier, Jerry
author_sort Galicia-Vázquez, Gabriela
collection PubMed
description MicroRNAs (miRNAs) are short noncoding RNAs that regulate gene expression through partial complementary base-pairing to the 3′ untranslated region (UTR) of target mRNAs. Inhibition of translation initiation has been identified as an early event of miRNA-mediated gene repression, but the underlying mechanistic details of this process are not well understood. Recently, eukaryotic initiation factor (eIF) 4AII was identified as a critical modulator of miRNA activity with depletion of this factor alleviating miRNA-mediated gene repression. Using the CRISPR/Cas9-editing system, we generated a novel cell line in which expression of eIF4AII was eliminated. The absence of eIF4AII does not affect cell viability, proliferation, or global mRNA translation. Importantly, we show that eIF4AII is dispensable for miRNA-mediated gene silencing.
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spelling pubmed-45747582016-10-01 eIF4AII is dispensable for miRNA-mediated gene silencing Galicia-Vázquez, Gabriela Chu, Jennifer Pelletier, Jerry RNA Article MicroRNAs (miRNAs) are short noncoding RNAs that regulate gene expression through partial complementary base-pairing to the 3′ untranslated region (UTR) of target mRNAs. Inhibition of translation initiation has been identified as an early event of miRNA-mediated gene repression, but the underlying mechanistic details of this process are not well understood. Recently, eukaryotic initiation factor (eIF) 4AII was identified as a critical modulator of miRNA activity with depletion of this factor alleviating miRNA-mediated gene repression. Using the CRISPR/Cas9-editing system, we generated a novel cell line in which expression of eIF4AII was eliminated. The absence of eIF4AII does not affect cell viability, proliferation, or global mRNA translation. Importantly, we show that eIF4AII is dispensable for miRNA-mediated gene silencing. Cold Spring Harbor Laboratory Press 2015-10 /pmc/articles/PMC4574758/ /pubmed/26286746 http://dx.doi.org/10.1261/rna.052225.115 Text en © 2015 Galicia-Vázquez et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Article
Galicia-Vázquez, Gabriela
Chu, Jennifer
Pelletier, Jerry
eIF4AII is dispensable for miRNA-mediated gene silencing
title eIF4AII is dispensable for miRNA-mediated gene silencing
title_full eIF4AII is dispensable for miRNA-mediated gene silencing
title_fullStr eIF4AII is dispensable for miRNA-mediated gene silencing
title_full_unstemmed eIF4AII is dispensable for miRNA-mediated gene silencing
title_short eIF4AII is dispensable for miRNA-mediated gene silencing
title_sort eif4aii is dispensable for mirna-mediated gene silencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574758/
https://www.ncbi.nlm.nih.gov/pubmed/26286746
http://dx.doi.org/10.1261/rna.052225.115
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