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eIF4AII is dispensable for miRNA-mediated gene silencing
MicroRNAs (miRNAs) are short noncoding RNAs that regulate gene expression through partial complementary base-pairing to the 3′ untranslated region (UTR) of target mRNAs. Inhibition of translation initiation has been identified as an early event of miRNA-mediated gene repression, but the underlying m...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574758/ https://www.ncbi.nlm.nih.gov/pubmed/26286746 http://dx.doi.org/10.1261/rna.052225.115 |
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author | Galicia-Vázquez, Gabriela Chu, Jennifer Pelletier, Jerry |
author_facet | Galicia-Vázquez, Gabriela Chu, Jennifer Pelletier, Jerry |
author_sort | Galicia-Vázquez, Gabriela |
collection | PubMed |
description | MicroRNAs (miRNAs) are short noncoding RNAs that regulate gene expression through partial complementary base-pairing to the 3′ untranslated region (UTR) of target mRNAs. Inhibition of translation initiation has been identified as an early event of miRNA-mediated gene repression, but the underlying mechanistic details of this process are not well understood. Recently, eukaryotic initiation factor (eIF) 4AII was identified as a critical modulator of miRNA activity with depletion of this factor alleviating miRNA-mediated gene repression. Using the CRISPR/Cas9-editing system, we generated a novel cell line in which expression of eIF4AII was eliminated. The absence of eIF4AII does not affect cell viability, proliferation, or global mRNA translation. Importantly, we show that eIF4AII is dispensable for miRNA-mediated gene silencing. |
format | Online Article Text |
id | pubmed-4574758 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-45747582016-10-01 eIF4AII is dispensable for miRNA-mediated gene silencing Galicia-Vázquez, Gabriela Chu, Jennifer Pelletier, Jerry RNA Article MicroRNAs (miRNAs) are short noncoding RNAs that regulate gene expression through partial complementary base-pairing to the 3′ untranslated region (UTR) of target mRNAs. Inhibition of translation initiation has been identified as an early event of miRNA-mediated gene repression, but the underlying mechanistic details of this process are not well understood. Recently, eukaryotic initiation factor (eIF) 4AII was identified as a critical modulator of miRNA activity with depletion of this factor alleviating miRNA-mediated gene repression. Using the CRISPR/Cas9-editing system, we generated a novel cell line in which expression of eIF4AII was eliminated. The absence of eIF4AII does not affect cell viability, proliferation, or global mRNA translation. Importantly, we show that eIF4AII is dispensable for miRNA-mediated gene silencing. Cold Spring Harbor Laboratory Press 2015-10 /pmc/articles/PMC4574758/ /pubmed/26286746 http://dx.doi.org/10.1261/rna.052225.115 Text en © 2015 Galicia-Vázquez et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Article Galicia-Vázquez, Gabriela Chu, Jennifer Pelletier, Jerry eIF4AII is dispensable for miRNA-mediated gene silencing |
title | eIF4AII is dispensable for miRNA-mediated gene silencing |
title_full | eIF4AII is dispensable for miRNA-mediated gene silencing |
title_fullStr | eIF4AII is dispensable for miRNA-mediated gene silencing |
title_full_unstemmed | eIF4AII is dispensable for miRNA-mediated gene silencing |
title_short | eIF4AII is dispensable for miRNA-mediated gene silencing |
title_sort | eif4aii is dispensable for mirna-mediated gene silencing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574758/ https://www.ncbi.nlm.nih.gov/pubmed/26286746 http://dx.doi.org/10.1261/rna.052225.115 |
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