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A Unique Primer with an Inosine Chain at the 5′-Terminus Improves the Reliability of SNP Analysis Using the PCR-Amplified Product Length Polymorphism Method

Polymerase chain reaction-amplified product length polymorphism (PCR-APLP) is one of the most convenient and reliable methods for single nucleotide polymorphism (SNP) analysis. This method is based on PCR, but uses allele-specific primers containing SNP sites at the 3′-terminus of each primer. To us...

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Detalles Bibliográficos
Autores principales: Shojo, Hideki, Tanaka, Mayumi, Takahashi, Ryohei, Kakuda, Tsuneo, Adachi, Noboru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575067/
https://www.ncbi.nlm.nih.gov/pubmed/26381262
http://dx.doi.org/10.1371/journal.pone.0136995