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A Unique Primer with an Inosine Chain at the 5′-Terminus Improves the Reliability of SNP Analysis Using the PCR-Amplified Product Length Polymorphism Method
Polymerase chain reaction-amplified product length polymorphism (PCR-APLP) is one of the most convenient and reliable methods for single nucleotide polymorphism (SNP) analysis. This method is based on PCR, but uses allele-specific primers containing SNP sites at the 3′-terminus of each primer. To us...
Autores principales: | Shojo, Hideki, Tanaka, Mayumi, Takahashi, Ryohei, Kakuda, Tsuneo, Adachi, Noboru |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575067/ https://www.ncbi.nlm.nih.gov/pubmed/26381262 http://dx.doi.org/10.1371/journal.pone.0136995 |
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