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Changes in Brain Metallome/Metabolome Pattern due to a Single i.v. Injection of Manganese in Rats

Exposure to high concentrations of Manganese (Mn) is known to potentially induce an accumulation in the brain, leading to a Parkinson related disease, called manganism. Versatile mechanisms of Mn-induced brain injury are discussed, with inactivation of mitochondrial defense against oxidative stress...

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Autores principales: Neth, Katharina, Lucio, Marianna, Walker, Alesia, Zorn, Julia, Schmitt-Kopplin, Philippe, Michalke, Bernhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575095/
https://www.ncbi.nlm.nih.gov/pubmed/26383269
http://dx.doi.org/10.1371/journal.pone.0138270
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author Neth, Katharina
Lucio, Marianna
Walker, Alesia
Zorn, Julia
Schmitt-Kopplin, Philippe
Michalke, Bernhard
author_facet Neth, Katharina
Lucio, Marianna
Walker, Alesia
Zorn, Julia
Schmitt-Kopplin, Philippe
Michalke, Bernhard
author_sort Neth, Katharina
collection PubMed
description Exposure to high concentrations of Manganese (Mn) is known to potentially induce an accumulation in the brain, leading to a Parkinson related disease, called manganism. Versatile mechanisms of Mn-induced brain injury are discussed, with inactivation of mitochondrial defense against oxidative stress being a major one. So far, studies indicate that the main Mn-species entering the brain are low molecular mass (LMM) compounds such as Mn-citrate. Applying a single low dose MnCl(2) injection in rats, we observed alterations in Mn-species pattern within the brain by analysis of aqueous brain extracts by size-exclusion chromatography—inductively coupled plasma mass spectrometry (SEC-ICP-MS). Additionally, electrospray ionization—ion cyclotron resonance-Fourier transform-mass spectrometry (ESI-ICR/FT-MS) measurement of methanolic brain extracts revealed a comprehensive analysis of changes in brain metabolisms after the single MnCl(2) injection. Major alterations were observed for amino acid, fatty acid, glutathione, glucose and purine/pyrimidine metabolism. The power of this metabolomic approach is the broad and detailed overview of affected brain metabolisms. We also correlated results from the metallomic investigations (Mn concentrations and Mn-species in brain) with the findings from metabolomics. This strategy might help to unravel the role of different Mn-species during Mn-induced alterations in brain metabolism.
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spelling pubmed-45750952015-09-25 Changes in Brain Metallome/Metabolome Pattern due to a Single i.v. Injection of Manganese in Rats Neth, Katharina Lucio, Marianna Walker, Alesia Zorn, Julia Schmitt-Kopplin, Philippe Michalke, Bernhard PLoS One Research Article Exposure to high concentrations of Manganese (Mn) is known to potentially induce an accumulation in the brain, leading to a Parkinson related disease, called manganism. Versatile mechanisms of Mn-induced brain injury are discussed, with inactivation of mitochondrial defense against oxidative stress being a major one. So far, studies indicate that the main Mn-species entering the brain are low molecular mass (LMM) compounds such as Mn-citrate. Applying a single low dose MnCl(2) injection in rats, we observed alterations in Mn-species pattern within the brain by analysis of aqueous brain extracts by size-exclusion chromatography—inductively coupled plasma mass spectrometry (SEC-ICP-MS). Additionally, electrospray ionization—ion cyclotron resonance-Fourier transform-mass spectrometry (ESI-ICR/FT-MS) measurement of methanolic brain extracts revealed a comprehensive analysis of changes in brain metabolisms after the single MnCl(2) injection. Major alterations were observed for amino acid, fatty acid, glutathione, glucose and purine/pyrimidine metabolism. The power of this metabolomic approach is the broad and detailed overview of affected brain metabolisms. We also correlated results from the metallomic investigations (Mn concentrations and Mn-species in brain) with the findings from metabolomics. This strategy might help to unravel the role of different Mn-species during Mn-induced alterations in brain metabolism. Public Library of Science 2015-09-18 /pmc/articles/PMC4575095/ /pubmed/26383269 http://dx.doi.org/10.1371/journal.pone.0138270 Text en © 2015 Neth et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Neth, Katharina
Lucio, Marianna
Walker, Alesia
Zorn, Julia
Schmitt-Kopplin, Philippe
Michalke, Bernhard
Changes in Brain Metallome/Metabolome Pattern due to a Single i.v. Injection of Manganese in Rats
title Changes in Brain Metallome/Metabolome Pattern due to a Single i.v. Injection of Manganese in Rats
title_full Changes in Brain Metallome/Metabolome Pattern due to a Single i.v. Injection of Manganese in Rats
title_fullStr Changes in Brain Metallome/Metabolome Pattern due to a Single i.v. Injection of Manganese in Rats
title_full_unstemmed Changes in Brain Metallome/Metabolome Pattern due to a Single i.v. Injection of Manganese in Rats
title_short Changes in Brain Metallome/Metabolome Pattern due to a Single i.v. Injection of Manganese in Rats
title_sort changes in brain metallome/metabolome pattern due to a single i.v. injection of manganese in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575095/
https://www.ncbi.nlm.nih.gov/pubmed/26383269
http://dx.doi.org/10.1371/journal.pone.0138270
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