A novel method to analyze 5-hydroxymethylcytosine in CpG sequences using maintenance DNA methyltransferase, DNMT1

Hydroxymethylcytosine has been shown to be involved in DNA demethylation and gene expression. Although methods to determine the position of hydroxymethylcytosine at single-base resolution have been reported, these methods involve some difficulties. Here, we report a simple method to analyze hydroxym...

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Detalles Bibliográficos
Autores principales: Takahashi, Saori, Suetake, Isao, Engelhardt, Jan, Tajima, Shoji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575954/
https://www.ncbi.nlm.nih.gov/pubmed/26504739
http://dx.doi.org/10.1016/j.fob.2015.09.003
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author Takahashi, Saori
Suetake, Isao
Engelhardt, Jan
Tajima, Shoji
author_facet Takahashi, Saori
Suetake, Isao
Engelhardt, Jan
Tajima, Shoji
author_sort Takahashi, Saori
collection PubMed
description Hydroxymethylcytosine has been shown to be involved in DNA demethylation and gene expression. Although methods to determine the position of hydroxymethylcytosine at single-base resolution have been reported, these methods involve some difficulties. Here, we report a simple method to analyze hydroxymethylcytosine in the CpG sequence utilizing the maintenance DNA methylation activity of DNMT1, which selectively methylates hemi-methylated but not hemi-hydroxymethylated CpG sequences. The method enables monitoring of the dynamics of the hydroxymethylation state of a specific genome site.
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spelling pubmed-45759542015-10-26 A novel method to analyze 5-hydroxymethylcytosine in CpG sequences using maintenance DNA methyltransferase, DNMT1 Takahashi, Saori Suetake, Isao Engelhardt, Jan Tajima, Shoji FEBS Open Bio Method Hydroxymethylcytosine has been shown to be involved in DNA demethylation and gene expression. Although methods to determine the position of hydroxymethylcytosine at single-base resolution have been reported, these methods involve some difficulties. Here, we report a simple method to analyze hydroxymethylcytosine in the CpG sequence utilizing the maintenance DNA methylation activity of DNMT1, which selectively methylates hemi-methylated but not hemi-hydroxymethylated CpG sequences. The method enables monitoring of the dynamics of the hydroxymethylation state of a specific genome site. Elsevier 2015-09-08 /pmc/articles/PMC4575954/ /pubmed/26504739 http://dx.doi.org/10.1016/j.fob.2015.09.003 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Method
Takahashi, Saori
Suetake, Isao
Engelhardt, Jan
Tajima, Shoji
A novel method to analyze 5-hydroxymethylcytosine in CpG sequences using maintenance DNA methyltransferase, DNMT1
title A novel method to analyze 5-hydroxymethylcytosine in CpG sequences using maintenance DNA methyltransferase, DNMT1
title_full A novel method to analyze 5-hydroxymethylcytosine in CpG sequences using maintenance DNA methyltransferase, DNMT1
title_fullStr A novel method to analyze 5-hydroxymethylcytosine in CpG sequences using maintenance DNA methyltransferase, DNMT1
title_full_unstemmed A novel method to analyze 5-hydroxymethylcytosine in CpG sequences using maintenance DNA methyltransferase, DNMT1
title_short A novel method to analyze 5-hydroxymethylcytosine in CpG sequences using maintenance DNA methyltransferase, DNMT1
title_sort novel method to analyze 5-hydroxymethylcytosine in cpg sequences using maintenance dna methyltransferase, dnmt1
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575954/
https://www.ncbi.nlm.nih.gov/pubmed/26504739
http://dx.doi.org/10.1016/j.fob.2015.09.003
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