Ultrasensitive single-nucleotide polymorphism detection using target-recycled ligation, strand displacement and enzymatic amplification

We report herein the development of a highly sensitive and selective approach for label-free DNA detection by combining target-recycled ligation (TRL), magnetic nanoparticle assisted target capture/separation, and efficient enzymatic amplification. We show that our approach can detect as little as 3...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Yue, Guo, Yuan, Quirke, Philip, Zhou, Dejian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2013
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576341/
https://www.ncbi.nlm.nih.gov/pubmed/23636707
http://dx.doi.org/10.1039/c3nr01010d

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576341/
https://www.ncbi.nlm.nih.gov/pubmed/23636707
http://dx.doi.org/10.1039/c3nr01010d

Ejemplares similares