Monodisperse Picoliter Droplets for Low-Bias and Contamination-Free Reactions in Single-Cell Whole Genome Amplification

Whole genome amplification (WGA) is essential for obtaining genome sequences from single bacterial cells because the quantity of template DNA contained in a single cell is very low. Multiple displacement amplification (MDA), using Phi29 DNA polymerase and random primers, is the most widely used meth...

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Detalles Bibliográficos
Autores principales: Nishikawa, Yohei, Hosokawa, Masahito, Maruyama, Toru, Yamagishi, Keisuke, Mori, Tetsushi, Takeyama, Haruko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4577099/
https://www.ncbi.nlm.nih.gov/pubmed/26389587
http://dx.doi.org/10.1371/journal.pone.0138733

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4577099/
https://www.ncbi.nlm.nih.gov/pubmed/26389587
http://dx.doi.org/10.1371/journal.pone.0138733

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