Cargando…

Monodisperse Picoliter Droplets for Low-Bias and Contamination-Free Reactions in Single-Cell Whole Genome Amplification

Whole genome amplification (WGA) is essential for obtaining genome sequences from single bacterial cells because the quantity of template DNA contained in a single cell is very low. Multiple displacement amplification (MDA), using Phi29 DNA polymerase and random primers, is the most widely used meth...

Descripción completa

Detalles Bibliográficos
Autores principales:	Nishikawa, Yohei, Hosokawa, Masahito, Maruyama, Toru, Yamagishi, Keisuke, Mori, Tetsushi, Takeyama, Haruko
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4577099/ https://www.ncbi.nlm.nih.gov/pubmed/26389587 http://dx.doi.org/10.1371/journal.pone.0138733

Ejemplares similares

Massively parallel whole genome amplification for single-cell sequencing using droplet microfluidics
por: Hosokawa, Masahito, et al.
Publicado: (2017)

SAG-QC: quality control of single amplified genome information by subtracting non-target sequences based on sequence compositions
por: Maruyama, Toru, et al.
Publicado: (2017)

Electrofusion of single cells in picoliter droplets
por: Schoeman, Rogier M., et al.
Publicado: (2018)

Obtaining high-quality draft genomes from uncultured microbes by cleaning and co-assembly of single-cell amplified genomes
por: Kogawa, Masato, et al.
Publicado: (2018)

Optically controlled coalescence and splitting of femtoliter/picoliter droplets for microreactors
por: Wen, Mingcong, et al.
Publicado: (2022)

Integration of Droplet Microfluidic Tools for Single-cell Functional Metagenomics: An Engineering Head Start
por: Conchouso, David, et al.
Publicado: (2021)

Macromolecular crowding develops heterogeneous environments of gene expression in picoliter droplets
por: Hansen, Maike M. K., et al.
Publicado: (2015)

One step DNA amplification of mammalian cells in picoliter microwell arrays
por: Liu, Wenwen, et al.
Publicado: (2019)

Picoliter droplet array based on bioinspired microholes for in situ single-cell analysis
por: Du, Lin, et al.
Publicado: (2020)

High-throughput identification of peptide agonists against GPCRs by co-culture of mammalian reporter cells and peptide-secreting yeast cells using droplet microfluidics
por: Yaginuma, Kenshi, et al.
Publicado: (2019)

In Situ Detection of Antibiotic Amphotericin B Produced in Streptomyces nodosus Using Raman Microspectroscopy
por: Miyaoka, Rimi, et al.
Publicado: (2014)

Massively parallel single-cell genomics of microbiomes in rice paddies
por: Aoki, Wataru, et al.
Publicado: (2022)

Multiplex Detection of Rare Mutations by Picoliter Droplet Based Digital PCR: Sensitivity and Specificity Considerations
por: Zonta, Eleonora, et al.
Publicado: (2016)

Using picoliter droplet deposition to track clonal competition in adherent and organoid cancer cell cultures
por: Baglamis, Selami, et al.
Publicado: (2023)

Revealing within-species diversity in uncultured human gut bacteria with single-cell long-read sequencing
por: Kogawa, Masato, et al.
Publicado: (2023)

Picoliter Droplet Generation and Dense Bead-in-Droplet Encapsulation via Microfluidic Devices Fabricated via 3D Printed Molds
por: Anyaduba, Tochukwu D., et al.
Publicado: (2022)

Attomole-Level Multiplexed Detection of Neurochemicals in Picoliter Droplets by On-Chip Nanoelectrospray Ionization Coupled to Mass Spectrometry
por: Zhang, Yan, et al.
Publicado: (2022)

A CCR5(+) memory subset within HIV-1-infected primary resting CD4(+) T cells is permissive for replication-competent, latently infected viruses in vitro
por: Terahara, Kazutaka, et al.
Publicado: (2019)

Correction to: A CCR5(+) memory subset within HIV-1-infected primary resting CD4(+) T cells is permissive for replication-competent, latently infected viruses in vitro
por: Terahara, Kazutaka, et al.
Publicado: (2019)

Spray Mode and Monodisperse Droplet Properties of an Electrospray
por: Kim, Ji Yeop, et al.
Publicado: (2022)

Single-cell genomics of uncultured bacteria reveals dietary fiber responders in the mouse gut microbiota
por: Chijiiwa, Rieka, et al.
Publicado: (2020)

Picoliter Well Array Chip-Based Digital Recombinase Polymerase Amplification for Absolute Quantification of Nucleic Acids
por: Li, Zhao, et al.
Publicado: (2016)

Enrichment of bacteria and alginate lyase genes potentially involved in brown alga degradation in the gut of marine gastropods
por: Ito, Michihiro, et al.
Publicado: (2019)

Integration of monolithic porous polymer with droplet-based microfluidics on a chip for nano/picoliter volume sample analysis
por: Kim, Jin-young, et al.
Publicado: (2014)

Accessing individual 75-micron diameter nozzles of a desktop inkjet printer to dispense picoliter droplets on demand
por: Waasdorp, Rick, et al.
Publicado: (2018)

Strain-level profiling of viable microbial community by selective single-cell genome sequencing
por: Hosokawa, Masahito, et al.
Publicado: (2022)

Formation and Elimination of Satellite Droplets during Monodisperse Droplet Generation by Using Piezoelectric Method
por: Hu, Zejian, et al.
Publicado: (2021)

Single-cell metabolite detection and genomics reveals uncultivated talented producer
por: Kogawa, Masato, et al.
Publicado: (2022)

Study on the Technology of Monodisperse Droplets by a High-Throughput and Instant-Mixing Droplet Microfluidic System
por: Xu, Rui, et al.
Publicado: (2021)

The effect of viscosity and surface tension on inkjet printed picoliter dots
por: Krainer, Sarah, et al.
Publicado: (2019)

Targeted single-cell genomics reveals novel host adaptation strategies of the symbiotic bacteria Endozoicomonas in Acropora tenuis coral
por: Ide, Keigo, et al.
Publicado: (2022)

Draft Genome Sequence of Okeania sp. Strain KiyG1, Assembled from Single-Amplified Genomes Collected from Cape Kiyan, Okinawa, Japan
por: Lewaru, Muhammad Wahyudin, et al.
Publicado: (2020)

Validation of the application of gel beads-based single-cell genome sequencing platform to soil and seawater
por: Nishikawa, Yohei, et al.
Publicado: (2022)

A hand-held, power-free microfluidic device for monodisperse droplet generation
por: Chen, I-Jane, et al.
Publicado: (2018)

Monodisperse Micro-Droplet Generation in Microfluidic Channel with Asymmetric Cross-Sectional Shape
por: Cho, Youngseo, et al.
Publicado: (2023)

Nanofluidic chips for cryo-EM structure determination from picoliter sample volumes
por: Huber, Stefan T, et al.
Publicado: (2022)

Monodisperse droplet formation by spontaneous and interaction based mechanisms in partitioned EDGE microfluidic device
por: ten Klooster, S., et al.
Publicado: (2019)

High-Aspect-Ratio Microfluidic Channel with Parallelogram Cross-Section for Monodisperse Droplet Generation
por: Ji, Hyeonyeong, et al.
Publicado: (2022)

Site-specific gene expression analysis using an automated tissue micro-dissection punching system
por: Yoda, Takuya, et al.
Publicado: (2017)

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
por: Gruenberger, Alexander, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_g3de01fj8mf0q61r7sho9s57um