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REC-1 and HIM-5 distribute meiotic crossovers and function redundantly in meiotic double-strand break formation in Caenorhabditis elegans
The Caenorhabditis elegans gene rec-1 was the first genetic locus identified in metazoa to affect the distribution of meiotic crossovers along the chromosome. We report that rec-1 encodes a distant paralog of HIM-5, which was discovered by whole-genome sequencing and confirmed by multiple genome-edi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579353/ https://www.ncbi.nlm.nih.gov/pubmed/26385965 http://dx.doi.org/10.1101/gad.266056.115 |
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author | Chung, George Rose, Ann M. Petalcorin, Mark I.R. Martin, Julie S. Kessler, Zebulin Sanchez-Pulido, Luis Ponting, Chris P. Yanowitz, Judith L. Boulton, Simon J. |
author_facet | Chung, George Rose, Ann M. Petalcorin, Mark I.R. Martin, Julie S. Kessler, Zebulin Sanchez-Pulido, Luis Ponting, Chris P. Yanowitz, Judith L. Boulton, Simon J. |
author_sort | Chung, George |
collection | PubMed |
description | The Caenorhabditis elegans gene rec-1 was the first genetic locus identified in metazoa to affect the distribution of meiotic crossovers along the chromosome. We report that rec-1 encodes a distant paralog of HIM-5, which was discovered by whole-genome sequencing and confirmed by multiple genome-edited alleles. REC-1 is phosphorylated by cyclin-dependent kinase (CDK) in vitro, and mutation of the CDK consensus sites in REC-1 compromises meiotic crossover distribution in vivo. Unexpectedly, rec-1; him-5 double mutants are synthetic-lethal due to a defect in meiotic double-strand break formation. Thus, we uncovered an unexpected robustness to meiotic DSB formation and crossover positioning that is executed by HIM-5 and REC-1 and regulated by phosphorylation. |
format | Online Article Text |
id | pubmed-4579353 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-45793532015-09-29 REC-1 and HIM-5 distribute meiotic crossovers and function redundantly in meiotic double-strand break formation in Caenorhabditis elegans Chung, George Rose, Ann M. Petalcorin, Mark I.R. Martin, Julie S. Kessler, Zebulin Sanchez-Pulido, Luis Ponting, Chris P. Yanowitz, Judith L. Boulton, Simon J. Genes Dev Research Paper The Caenorhabditis elegans gene rec-1 was the first genetic locus identified in metazoa to affect the distribution of meiotic crossovers along the chromosome. We report that rec-1 encodes a distant paralog of HIM-5, which was discovered by whole-genome sequencing and confirmed by multiple genome-edited alleles. REC-1 is phosphorylated by cyclin-dependent kinase (CDK) in vitro, and mutation of the CDK consensus sites in REC-1 compromises meiotic crossover distribution in vivo. Unexpectedly, rec-1; him-5 double mutants are synthetic-lethal due to a defect in meiotic double-strand break formation. Thus, we uncovered an unexpected robustness to meiotic DSB formation and crossover positioning that is executed by HIM-5 and REC-1 and regulated by phosphorylation. Cold Spring Harbor Laboratory Press 2015-09-15 /pmc/articles/PMC4579353/ /pubmed/26385965 http://dx.doi.org/10.1101/gad.266056.115 Text en © 2015 Chung et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by/4.0/ This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Paper Chung, George Rose, Ann M. Petalcorin, Mark I.R. Martin, Julie S. Kessler, Zebulin Sanchez-Pulido, Luis Ponting, Chris P. Yanowitz, Judith L. Boulton, Simon J. REC-1 and HIM-5 distribute meiotic crossovers and function redundantly in meiotic double-strand break formation in Caenorhabditis elegans |
title | REC-1 and HIM-5 distribute meiotic crossovers and function redundantly in meiotic double-strand break formation in Caenorhabditis elegans |
title_full | REC-1 and HIM-5 distribute meiotic crossovers and function redundantly in meiotic double-strand break formation in Caenorhabditis elegans |
title_fullStr | REC-1 and HIM-5 distribute meiotic crossovers and function redundantly in meiotic double-strand break formation in Caenorhabditis elegans |
title_full_unstemmed | REC-1 and HIM-5 distribute meiotic crossovers and function redundantly in meiotic double-strand break formation in Caenorhabditis elegans |
title_short | REC-1 and HIM-5 distribute meiotic crossovers and function redundantly in meiotic double-strand break formation in Caenorhabditis elegans |
title_sort | rec-1 and him-5 distribute meiotic crossovers and function redundantly in meiotic double-strand break formation in caenorhabditis elegans |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579353/ https://www.ncbi.nlm.nih.gov/pubmed/26385965 http://dx.doi.org/10.1101/gad.266056.115 |
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