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Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues

B7-H3 protein is an important tumor antigen, but the expression of its isoforms, 4IgB7-H3 and 2IgB7-H3, in tumor tissues remains unknown due to the lack of specific monoclonal antibodies (mAbs). In the present study, a mAb (9C3) specifically recognizing 2IgB7-H3, but not 4IgB7-H3, was prepared. Usin...

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Detalles Bibliográficos
Autores principales: WANG, ZHENXIN, YANG, JIANFENG, ZHU, YANBO, ZHU, YUN, ZHANG, BIN, ZHOU, YINGHUI
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579917/
https://www.ncbi.nlm.nih.gov/pubmed/26622819
http://dx.doi.org/10.3892/ol.2015.3611
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author WANG, ZHENXIN
YANG, JIANFENG
ZHU, YANBO
ZHU, YUN
ZHANG, BIN
ZHOU, YINGHUI
author_facet WANG, ZHENXIN
YANG, JIANFENG
ZHU, YANBO
ZHU, YUN
ZHANG, BIN
ZHOU, YINGHUI
author_sort WANG, ZHENXIN
collection PubMed
description B7-H3 protein is an important tumor antigen, but the expression of its isoforms, 4IgB7-H3 and 2IgB7-H3, in tumor tissues remains unknown due to the lack of specific monoclonal antibodies (mAbs). In the present study, a mAb (9C3) specifically recognizing 2IgB7-H3, but not 4IgB7-H3, was prepared. Using 9C3 and a previously prepared mAb (4H7) that recognizes 4IgB7-H3 and 2IgB7-H3, the differential expression of 2IgB7-H3 and 4IgB7-H3 was analyzed in a variety of tumor cell lines by flow cytometry. It was found that 4IgB7-H3 had a more broad spectrum of expression among the cell lines compared with 2IgB7-H3. The expression of the two isoforms was further examined in glioma tissues using reverse transcription-polymerase chain reaction and immunohistochemistry techniques. The data revealed that 2IgB7-H3, but not 4IgB7-H3, was specifically expressed in glioma. Taken together, these results demonstrated for the first time that 2IgB7-H3 is a valuable biomarker for the diagnosis of glioma.
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spelling pubmed-45799172015-11-30 Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues WANG, ZHENXIN YANG, JIANFENG ZHU, YANBO ZHU, YUN ZHANG, BIN ZHOU, YINGHUI Oncol Lett Articles B7-H3 protein is an important tumor antigen, but the expression of its isoforms, 4IgB7-H3 and 2IgB7-H3, in tumor tissues remains unknown due to the lack of specific monoclonal antibodies (mAbs). In the present study, a mAb (9C3) specifically recognizing 2IgB7-H3, but not 4IgB7-H3, was prepared. Using 9C3 and a previously prepared mAb (4H7) that recognizes 4IgB7-H3 and 2IgB7-H3, the differential expression of 2IgB7-H3 and 4IgB7-H3 was analyzed in a variety of tumor cell lines by flow cytometry. It was found that 4IgB7-H3 had a more broad spectrum of expression among the cell lines compared with 2IgB7-H3. The expression of the two isoforms was further examined in glioma tissues using reverse transcription-polymerase chain reaction and immunohistochemistry techniques. The data revealed that 2IgB7-H3, but not 4IgB7-H3, was specifically expressed in glioma. Taken together, these results demonstrated for the first time that 2IgB7-H3 is a valuable biomarker for the diagnosis of glioma. D.A. Spandidos 2015-10 2015-08-14 /pmc/articles/PMC4579917/ /pubmed/26622819 http://dx.doi.org/10.3892/ol.2015.3611 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of a Creative Commons Attribution License. http://creativecommons.org/licenses/by/4.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
WANG, ZHENXIN
YANG, JIANFENG
ZHU, YANBO
ZHU, YUN
ZHANG, BIN
ZHOU, YINGHUI
Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues
title Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues
title_full Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues
title_fullStr Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues
title_full_unstemmed Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues
title_short Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues
title_sort differential expression of 2igb7-h3 and 4igb7-h3 in cancer cell lines and glioma tissues
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579917/
https://www.ncbi.nlm.nih.gov/pubmed/26622819
http://dx.doi.org/10.3892/ol.2015.3611
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