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Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues
B7-H3 protein is an important tumor antigen, but the expression of its isoforms, 4IgB7-H3 and 2IgB7-H3, in tumor tissues remains unknown due to the lack of specific monoclonal antibodies (mAbs). In the present study, a mAb (9C3) specifically recognizing 2IgB7-H3, but not 4IgB7-H3, was prepared. Usin...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579917/ https://www.ncbi.nlm.nih.gov/pubmed/26622819 http://dx.doi.org/10.3892/ol.2015.3611 |
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author | WANG, ZHENXIN YANG, JIANFENG ZHU, YANBO ZHU, YUN ZHANG, BIN ZHOU, YINGHUI |
author_facet | WANG, ZHENXIN YANG, JIANFENG ZHU, YANBO ZHU, YUN ZHANG, BIN ZHOU, YINGHUI |
author_sort | WANG, ZHENXIN |
collection | PubMed |
description | B7-H3 protein is an important tumor antigen, but the expression of its isoforms, 4IgB7-H3 and 2IgB7-H3, in tumor tissues remains unknown due to the lack of specific monoclonal antibodies (mAbs). In the present study, a mAb (9C3) specifically recognizing 2IgB7-H3, but not 4IgB7-H3, was prepared. Using 9C3 and a previously prepared mAb (4H7) that recognizes 4IgB7-H3 and 2IgB7-H3, the differential expression of 2IgB7-H3 and 4IgB7-H3 was analyzed in a variety of tumor cell lines by flow cytometry. It was found that 4IgB7-H3 had a more broad spectrum of expression among the cell lines compared with 2IgB7-H3. The expression of the two isoforms was further examined in glioma tissues using reverse transcription-polymerase chain reaction and immunohistochemistry techniques. The data revealed that 2IgB7-H3, but not 4IgB7-H3, was specifically expressed in glioma. Taken together, these results demonstrated for the first time that 2IgB7-H3 is a valuable biomarker for the diagnosis of glioma. |
format | Online Article Text |
id | pubmed-4579917 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-45799172015-11-30 Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues WANG, ZHENXIN YANG, JIANFENG ZHU, YANBO ZHU, YUN ZHANG, BIN ZHOU, YINGHUI Oncol Lett Articles B7-H3 protein is an important tumor antigen, but the expression of its isoforms, 4IgB7-H3 and 2IgB7-H3, in tumor tissues remains unknown due to the lack of specific monoclonal antibodies (mAbs). In the present study, a mAb (9C3) specifically recognizing 2IgB7-H3, but not 4IgB7-H3, was prepared. Using 9C3 and a previously prepared mAb (4H7) that recognizes 4IgB7-H3 and 2IgB7-H3, the differential expression of 2IgB7-H3 and 4IgB7-H3 was analyzed in a variety of tumor cell lines by flow cytometry. It was found that 4IgB7-H3 had a more broad spectrum of expression among the cell lines compared with 2IgB7-H3. The expression of the two isoforms was further examined in glioma tissues using reverse transcription-polymerase chain reaction and immunohistochemistry techniques. The data revealed that 2IgB7-H3, but not 4IgB7-H3, was specifically expressed in glioma. Taken together, these results demonstrated for the first time that 2IgB7-H3 is a valuable biomarker for the diagnosis of glioma. D.A. Spandidos 2015-10 2015-08-14 /pmc/articles/PMC4579917/ /pubmed/26622819 http://dx.doi.org/10.3892/ol.2015.3611 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of a Creative Commons Attribution License. http://creativecommons.org/licenses/by/4.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles WANG, ZHENXIN YANG, JIANFENG ZHU, YANBO ZHU, YUN ZHANG, BIN ZHOU, YINGHUI Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues |
title | Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues |
title_full | Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues |
title_fullStr | Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues |
title_full_unstemmed | Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues |
title_short | Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues |
title_sort | differential expression of 2igb7-h3 and 4igb7-h3 in cancer cell lines and glioma tissues |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579917/ https://www.ncbi.nlm.nih.gov/pubmed/26622819 http://dx.doi.org/10.3892/ol.2015.3611 |
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