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Thon rings from amorphous ice and implications of beam-induced Brownian motion in single particle electron cryo-microscopy

We have recorded dose-fractionated electron cryo-microscope images of thin films of pure flash-frozen amorphous ice and pre-irradiated amorphous carbon on a Falcon II direct electron detector using 300 keV electrons. We observe Thon rings [1] in both the power spectrum of the summed frames and the s...

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Detalles Bibliográficos
Autores principales: G. McMullan, Vinothkumar, K.R., Henderson, R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4584428/
https://www.ncbi.nlm.nih.gov/pubmed/26103047
http://dx.doi.org/10.1016/j.ultramic.2015.05.017