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Thon rings from amorphous ice and implications of beam-induced Brownian motion in single particle electron cryo-microscopy
We have recorded dose-fractionated electron cryo-microscope images of thin films of pure flash-frozen amorphous ice and pre-irradiated amorphous carbon on a Falcon II direct electron detector using 300 keV electrons. We observe Thon rings [1] in both the power spectrum of the summed frames and the s...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4584428/ https://www.ncbi.nlm.nih.gov/pubmed/26103047 http://dx.doi.org/10.1016/j.ultramic.2015.05.017 |