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Fast and Precise 3D Fluorophore Localization based on Gradient Fitting

Astigmatism imaging approach has been widely used to encode the fluorophore’s 3D position in single-particle tracking and super-resolution localization microscopy. Here, we present a new high-speed localization algorithm based on gradient fitting to precisely decode the 3D subpixel position of the f...

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Detalles Bibliográficos
Autores principales: Ma, Hongqiang, Xu, Jianquan, Jin, Jingyi, Gao, Ying, Lan, Li, Liu, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585720/
https://www.ncbi.nlm.nih.gov/pubmed/26390959
http://dx.doi.org/10.1038/srep14335