Cargando…

Optimization of neuronal cultures from rat superior cervical ganglia for dual patch recording

Superior cervical ganglion neurons (SCGN) are often used to investigate neurotransmitter release mechanisms. In this study, we optimized the dissociation and culture conditions of rat SCGN cultures for dual patch clamp recordings. Two weeks in vitro are sufficient to achieve a significant CNTF-induc...

Descripción completa

Detalles Bibliográficos
Autores principales: Amendola, Julien, Boumedine, Norah, Sangiardi, Marion, El Far, Oussama
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585864/
https://www.ncbi.nlm.nih.gov/pubmed/26399440
http://dx.doi.org/10.1038/srep14455