Optimization of neuronal cultures from rat superior cervical ganglia for dual patch recording

Superior cervical ganglion neurons (SCGN) are often used to investigate neurotransmitter release mechanisms. In this study, we optimized the dissociation and culture conditions of rat SCGN cultures for dual patch clamp recordings. Two weeks in vitro are sufficient to achieve a significant CNTF-induc...

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Autores principales: Amendola, Julien, Boumedine, Norah, Sangiardi, Marion, El Far, Oussama
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585864/
https://www.ncbi.nlm.nih.gov/pubmed/26399440
http://dx.doi.org/10.1038/srep14455
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author Amendola, Julien
Boumedine, Norah
Sangiardi, Marion
El Far, Oussama
author_facet Amendola, Julien
Boumedine, Norah
Sangiardi, Marion
El Far, Oussama
author_sort Amendola, Julien
collection PubMed
description Superior cervical ganglion neurons (SCGN) are often used to investigate neurotransmitter release mechanisms. In this study, we optimized the dissociation and culture conditions of rat SCGN cultures for dual patch clamp recordings. Two weeks in vitro are sufficient to achieve a significant CNTF-induced cholinergic switch and to develop mature and healthy neuronal profiles suited for detailed patch clamp analysis. One single pup provides sufficient material to prepare what was formerly obtained from 12 to 15 animals. The suitability of these cultures to study neurotransmitter release mechanisms was validated by presynaptically perturbing the interaction of the v-SNARE VAMP2 with the vesicular V-ATPase V0c subunit.
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spelling pubmed-45858642015-09-29 Optimization of neuronal cultures from rat superior cervical ganglia for dual patch recording Amendola, Julien Boumedine, Norah Sangiardi, Marion El Far, Oussama Sci Rep Article Superior cervical ganglion neurons (SCGN) are often used to investigate neurotransmitter release mechanisms. In this study, we optimized the dissociation and culture conditions of rat SCGN cultures for dual patch clamp recordings. Two weeks in vitro are sufficient to achieve a significant CNTF-induced cholinergic switch and to develop mature and healthy neuronal profiles suited for detailed patch clamp analysis. One single pup provides sufficient material to prepare what was formerly obtained from 12 to 15 animals. The suitability of these cultures to study neurotransmitter release mechanisms was validated by presynaptically perturbing the interaction of the v-SNARE VAMP2 with the vesicular V-ATPase V0c subunit. Nature Publishing Group 2015-09-24 /pmc/articles/PMC4585864/ /pubmed/26399440 http://dx.doi.org/10.1038/srep14455 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Amendola, Julien
Boumedine, Norah
Sangiardi, Marion
El Far, Oussama
Optimization of neuronal cultures from rat superior cervical ganglia for dual patch recording
title Optimization of neuronal cultures from rat superior cervical ganglia for dual patch recording
title_full Optimization of neuronal cultures from rat superior cervical ganglia for dual patch recording
title_fullStr Optimization of neuronal cultures from rat superior cervical ganglia for dual patch recording
title_full_unstemmed Optimization of neuronal cultures from rat superior cervical ganglia for dual patch recording
title_short Optimization of neuronal cultures from rat superior cervical ganglia for dual patch recording
title_sort optimization of neuronal cultures from rat superior cervical ganglia for dual patch recording
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585864/
https://www.ncbi.nlm.nih.gov/pubmed/26399440
http://dx.doi.org/10.1038/srep14455
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