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Structural and Antihypertensive Properties of Enzymatic Hemp Seed Protein Hydrolysates

The aim of this work was to produce antihypertensive protein hydrolysates through different forms of enzymatic hydrolysis (2% pepsin, 4% pepsin, 1% alcalase, 2% alcalase, 2% papain, and 2% pepsin + pancreatin) of hemp seed proteins (HSP). The hemp seed protein hydrolysates (HPHs) were tested for in...

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Autores principales: Malomo, Sunday A., Onuh, John O., Girgih, Abraham T., Aluko, Rotimi E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4586553/
https://www.ncbi.nlm.nih.gov/pubmed/26378569
http://dx.doi.org/10.3390/nu7095358
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author Malomo, Sunday A.
Onuh, John O.
Girgih, Abraham T.
Aluko, Rotimi E.
author_facet Malomo, Sunday A.
Onuh, John O.
Girgih, Abraham T.
Aluko, Rotimi E.
author_sort Malomo, Sunday A.
collection PubMed
description The aim of this work was to produce antihypertensive protein hydrolysates through different forms of enzymatic hydrolysis (2% pepsin, 4% pepsin, 1% alcalase, 2% alcalase, 2% papain, and 2% pepsin + pancreatin) of hemp seed proteins (HSP). The hemp seed protein hydrolysates (HPHs) were tested for in vitro inhibitions of renin and angiotensin-converting enzyme (ACE), two of the enzymes that regulate human blood pressure. The HPHs were then administered orally (200 mg/kg body weight) to spontaneously hypertensive rats and systolic blood pressure (SBP)-lowering effects measured over a 24 h period. Size exclusion chromatography mainly showed a 300–9560 Da peptide size range for the HPHs, while amino acid composition data had the 2% pepsin HPH with the highest cysteine content. Fluorescence spectroscopy revealed higher fluorescence intensities for the peptides when compared to the unhydrolyzed hemp seed protein. Overall, the 1% alcalase HPH was the most effective (p < 0.05) SBP-reducing agent (−32.5 ± 0.7 mmHg after 4 h), while the pepsin HPHs produced longer-lasting effects (−23.0 ± 1.4 mmHg after 24 h). We conclude that an optimized combination of the fast-acting HPH (1% alcalase) with the longer-lasting HPHs (2% and 4% pepsin) could provide daily effective SBP reductions.
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spelling pubmed-45865532015-10-06 Structural and Antihypertensive Properties of Enzymatic Hemp Seed Protein Hydrolysates Malomo, Sunday A. Onuh, John O. Girgih, Abraham T. Aluko, Rotimi E. Nutrients Article The aim of this work was to produce antihypertensive protein hydrolysates through different forms of enzymatic hydrolysis (2% pepsin, 4% pepsin, 1% alcalase, 2% alcalase, 2% papain, and 2% pepsin + pancreatin) of hemp seed proteins (HSP). The hemp seed protein hydrolysates (HPHs) were tested for in vitro inhibitions of renin and angiotensin-converting enzyme (ACE), two of the enzymes that regulate human blood pressure. The HPHs were then administered orally (200 mg/kg body weight) to spontaneously hypertensive rats and systolic blood pressure (SBP)-lowering effects measured over a 24 h period. Size exclusion chromatography mainly showed a 300–9560 Da peptide size range for the HPHs, while amino acid composition data had the 2% pepsin HPH with the highest cysteine content. Fluorescence spectroscopy revealed higher fluorescence intensities for the peptides when compared to the unhydrolyzed hemp seed protein. Overall, the 1% alcalase HPH was the most effective (p < 0.05) SBP-reducing agent (−32.5 ± 0.7 mmHg after 4 h), while the pepsin HPHs produced longer-lasting effects (−23.0 ± 1.4 mmHg after 24 h). We conclude that an optimized combination of the fast-acting HPH (1% alcalase) with the longer-lasting HPHs (2% and 4% pepsin) could provide daily effective SBP reductions. MDPI 2015-09-10 /pmc/articles/PMC4586553/ /pubmed/26378569 http://dx.doi.org/10.3390/nu7095358 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Malomo, Sunday A.
Onuh, John O.
Girgih, Abraham T.
Aluko, Rotimi E.
Structural and Antihypertensive Properties of Enzymatic Hemp Seed Protein Hydrolysates
title Structural and Antihypertensive Properties of Enzymatic Hemp Seed Protein Hydrolysates
title_full Structural and Antihypertensive Properties of Enzymatic Hemp Seed Protein Hydrolysates
title_fullStr Structural and Antihypertensive Properties of Enzymatic Hemp Seed Protein Hydrolysates
title_full_unstemmed Structural and Antihypertensive Properties of Enzymatic Hemp Seed Protein Hydrolysates
title_short Structural and Antihypertensive Properties of Enzymatic Hemp Seed Protein Hydrolysates
title_sort structural and antihypertensive properties of enzymatic hemp seed protein hydrolysates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4586553/
https://www.ncbi.nlm.nih.gov/pubmed/26378569
http://dx.doi.org/10.3390/nu7095358
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