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Expression of Recombinant pET22b-LysK-Cysteine/Histidine-Dependent Amidohydrolase/Peptidase Bacteriophage Therapeutic Protein in Escherichia coli BL21 (DE3)

OBJECTIVES: Bacteriophage-encoded endolysins are a group of enzymes that act by digesting the peptidoglycan of bacterial cell walls. LysK has been reported to lyse live staphylococcal cultures. LysK proteins containing only the cysteine/histidine-dependent amidohydrolase/peptidase (CHAP) domain has...

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Detalles Bibliográficos
Autores principales: Kashani, Hamed Haddad, Moniri, Rezvan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korea Centers for Disease Control and Prevention 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588433/
https://www.ncbi.nlm.nih.gov/pubmed/26473093
http://dx.doi.org/10.1016/j.phrp.2015.08.001