Determining exon connectivity in complex mRNAs by nanopore sequencing

Short-read high-throughput RNA sequencing, though powerful, is limited in its ability to directly measure exon connectivity in mRNAs that contain multiple alternative exons located farther apart than the maximum read length. Here, we use the Oxford Nanopore MinION sequencer to identify 7,899 ‘full-l...

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Detalles Bibliográficos
Autores principales: Bolisetty, Mohan T., Rajadinakaran, Gopinath, Graveley, Brenton R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588896/
https://www.ncbi.nlm.nih.gov/pubmed/26420219
http://dx.doi.org/10.1186/s13059-015-0777-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588896/
https://www.ncbi.nlm.nih.gov/pubmed/26420219
http://dx.doi.org/10.1186/s13059-015-0777-z

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