An Alternative Method to Facilitate cDNA Cloning for Expression Studies in Mammalian Cells by Introducing Positive Blue White Selection in Vaccinia Topoisomerase I-Mediated Recombination

One of the most basic techniques in biomedical research is cDNA cloning for expression studies in mammalian cells. Vaccinia topoisomerase I-mediated cloning (TOPO cloning by Invitrogen) allows fast and efficient recombination of PCR-amplified DNAs. Among TOPO vectors, a pcDNA3.1 directional cloning...

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Detalles Bibliográficos
Autor principal: Udo, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589362/
https://www.ncbi.nlm.nih.gov/pubmed/26422141
http://dx.doi.org/10.1371/journal.pone.0139349

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589362/
https://www.ncbi.nlm.nih.gov/pubmed/26422141
http://dx.doi.org/10.1371/journal.pone.0139349

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