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CRISPRscan: designing highly efficient sgRNAs for CRISPR/Cas9 targeting in vivo
CRISPR/Cas9 technology provides a powerful system for genome engineering. However, variable activity across different single guide RNAs (sgRNAs) remains a significant limitation. We have analyzed the molecular features that influence sgRNA stability, activity and loading into Cas9 in vivo. We observ...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589495/ https://www.ncbi.nlm.nih.gov/pubmed/26322839 http://dx.doi.org/10.1038/nmeth.3543 |