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CRISPRscan: designing highly efficient sgRNAs for CRISPR/Cas9 targeting in vivo

CRISPR/Cas9 technology provides a powerful system for genome engineering. However, variable activity across different single guide RNAs (sgRNAs) remains a significant limitation. We have analyzed the molecular features that influence sgRNA stability, activity and loading into Cas9 in vivo. We observ...

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Detalles Bibliográficos
Autores principales:	Moreno-Mateos, Miguel A., Vejnar, Charles E., Beaudoin, Jean-Denis, Fernandez, Juan P., Mis, Emily K., Khokha, Mustafa K., Giraldez, Antonio J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2015
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589495/ https://www.ncbi.nlm.nih.gov/pubmed/26322839 http://dx.doi.org/10.1038/nmeth.3543

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