Fluorescent derivatization of a protease antigen to track antigen uptake and processing in human cell lines

BACKGROUND: We have devised a simple and efficient fluorescence-based method to track antigen uptake and processing in human B lymphoblastoid cells (B-LCL). Fluorescein labelled subtilisin was used to optimize antigen uptake conditions and identify processed peptides from human cell lines. RESULTS:...

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Detalles Bibliográficos
Autores principales: Patil, Namrata S, Wong, David L, Collier, Katherine D, McDonald, Hugh C
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC459215/
https://www.ncbi.nlm.nih.gov/pubmed/15222895
http://dx.doi.org/10.1186/1471-2172-5-12

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC459215/
https://www.ncbi.nlm.nih.gov/pubmed/15222895
http://dx.doi.org/10.1186/1471-2172-5-12

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