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Fluorescent derivatization of a protease antigen to track antigen uptake and processing in human cell lines
BACKGROUND: We have devised a simple and efficient fluorescence-based method to track antigen uptake and processing in human B lymphoblastoid cells (B-LCL). Fluorescein labelled subtilisin was used to optimize antigen uptake conditions and identify processed peptides from human cell lines. RESULTS:...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2004
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC459215/ https://www.ncbi.nlm.nih.gov/pubmed/15222895 http://dx.doi.org/10.1186/1471-2172-5-12 |