Direct injection of cell-free Kir1.1 protein into Xenopus oocytes replicates single-channel currents derived from Kir1.1 mRNA

The development of integral membrane protein cell-free synthesis permits in-vitro labeling of accessible cysteines for real-time FRET and LRET measurements. The functional integrity of these synthetic ion channel proteins has been verified at the whole oocyte level by direct injection into, and reco...

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Detalles Bibliográficos
Autores principales: Sackin, Henry, Nanazashvili, Mikheil, Makino, Shin-ichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2015
Materias:
Technical Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594486/
https://www.ncbi.nlm.nih.gov/pubmed/26102359
http://dx.doi.org/10.1080/19336950.2015.1063752

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594486/
https://www.ncbi.nlm.nih.gov/pubmed/26102359
http://dx.doi.org/10.1080/19336950.2015.1063752

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