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mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes

Insulin stimulates the delivery of glucose transporter-4 (GLUT4)-containing vesicles to the surface of adipocytes. Depletion of the Sec1/Munc18 protein mVps45 significantly abrogates insulin-stimulated glucose transport and GLUT4 translocation. Here we show that depletion of mVps45 selectively reduc...

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Autores principales: Sadler, Jessica B A, Roccisana, Jennifer, Virolainen, Minttu, Bryant, Nia J, Gould, Gwyn W
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594494/
https://www.ncbi.nlm.nih.gov/pubmed/26479872
http://dx.doi.org/10.1080/19420889.2015.1026494
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author Sadler, Jessica B A
Roccisana, Jennifer
Virolainen, Minttu
Bryant, Nia J
Gould, Gwyn W
author_facet Sadler, Jessica B A
Roccisana, Jennifer
Virolainen, Minttu
Bryant, Nia J
Gould, Gwyn W
author_sort Sadler, Jessica B A
collection PubMed
description Insulin stimulates the delivery of glucose transporter-4 (GLUT4)-containing vesicles to the surface of adipocytes. Depletion of the Sec1/Munc18 protein mVps45 significantly abrogates insulin-stimulated glucose transport and GLUT4 translocation. Here we show that depletion of mVps45 selectively reduced expression of VAMPs 2 and 4, but not other VAMP isoforms. Although we did not observe direct interaction of mVps45 with any VAMP isoform; we found that the cognate binding partner of mVps45, Syntaxin 16 associates with VAMPs 2, 4, 7 and 8 in vitro. Co-immunoprecipitation experiments in 3T3-L1 adipocytes revealed an interaction between Syntaxin 16 and only VAMP4. We suggest GLUT4 trafficking is controlled by the coordinated expression of mVps45/Syntaxin 16/VAMP4, and that depletion of mVps45 regulates VAMP2 levels indirectly, perhaps via reduced trafficking into specialized subcellular compartments.
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spelling pubmed-45944942015-10-16 mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes Sadler, Jessica B A Roccisana, Jennifer Virolainen, Minttu Bryant, Nia J Gould, Gwyn W Commun Integr Biol Article Addendum Insulin stimulates the delivery of glucose transporter-4 (GLUT4)-containing vesicles to the surface of adipocytes. Depletion of the Sec1/Munc18 protein mVps45 significantly abrogates insulin-stimulated glucose transport and GLUT4 translocation. Here we show that depletion of mVps45 selectively reduced expression of VAMPs 2 and 4, but not other VAMP isoforms. Although we did not observe direct interaction of mVps45 with any VAMP isoform; we found that the cognate binding partner of mVps45, Syntaxin 16 associates with VAMPs 2, 4, 7 and 8 in vitro. Co-immunoprecipitation experiments in 3T3-L1 adipocytes revealed an interaction between Syntaxin 16 and only VAMP4. We suggest GLUT4 trafficking is controlled by the coordinated expression of mVps45/Syntaxin 16/VAMP4, and that depletion of mVps45 regulates VAMP2 levels indirectly, perhaps via reduced trafficking into specialized subcellular compartments. Taylor & Francis 2015-06-24 /pmc/articles/PMC4594494/ /pubmed/26479872 http://dx.doi.org/10.1080/19420889.2015.1026494 Text en © 2015 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Article Addendum
Sadler, Jessica B A
Roccisana, Jennifer
Virolainen, Minttu
Bryant, Nia J
Gould, Gwyn W
mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes
title mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes
title_full mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes
title_fullStr mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes
title_full_unstemmed mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes
title_short mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes
title_sort mvps45 knockdown selectively modulates vamp expression in 3t3-l1 adipocytes
topic Article Addendum
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594494/
https://www.ncbi.nlm.nih.gov/pubmed/26479872
http://dx.doi.org/10.1080/19420889.2015.1026494
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