Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes

Use of the CRISPR/Cas9 RNA-guided endonuclease complex has recently enabled the generation of double-strand breaks virtually anywhere in the C. elegans genome. Here, we present an improved strategy that makes all steps in the genome editing process more efficient. We have created a toolkit of templa...

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Detalles Bibliográficos
Autores principales: Norris, Adam D., Kim, Hyun-Min, Colaiácovo, Mónica P., Calarco, John A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2015
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4596661/
https://www.ncbi.nlm.nih.gov/pubmed/26232410
http://dx.doi.org/10.1534/genetics.115.180679

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4596661/
https://www.ncbi.nlm.nih.gov/pubmed/26232410
http://dx.doi.org/10.1534/genetics.115.180679

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