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Synthesis of an arrayed sgRNA library targeting the human genome
Clustered regularly interspaced short palindromic repeats (CRISPR) in conjunction with CRISPR-associated proteins (Cas) can be employed to introduce double stand breaks into mammalian genomes at user-defined loci. The endonuclease activity of the Cas complex can be targeted to a specific genomic reg...
Autores principales: | Schmidt, Tobias, Schmid-Burgk, Jonathan L., Hornung, Veit |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4597219/ https://www.ncbi.nlm.nih.gov/pubmed/26446710 http://dx.doi.org/10.1038/srep14987 |
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