Cargando…
Effects of Concentration and Reaction Time of Trypsin, Pepsin, and Chymotrypsin on the Hydrolysis Efficiency of Porcine Placenta
This study investigated the effects of three proteases (trypsin, pepsin and chymotrypsin) on the hydrolysis efficiency of porcine placenta and the molecular weight (Mw) distributions of the placental hydrolysates. Because placenta was made up of insoluble collagen, the placenta was gelatinized by ap...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Food Science of Animal Resources
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4597853/ https://www.ncbi.nlm.nih.gov/pubmed/26760932 http://dx.doi.org/10.5851/kosfa.2014.34.2.151 |
_version_ | 1782394009844449280 |
---|---|
author | Jung, Kyung-Hun Choi, Ye-Chul Chun, Ji-Yeon Min, Sang-Gi Hong, Geun-Pyo |
author_facet | Jung, Kyung-Hun Choi, Ye-Chul Chun, Ji-Yeon Min, Sang-Gi Hong, Geun-Pyo |
author_sort | Jung, Kyung-Hun |
collection | PubMed |
description | This study investigated the effects of three proteases (trypsin, pepsin and chymotrypsin) on the hydrolysis efficiency of porcine placenta and the molecular weight (Mw) distributions of the placental hydrolysates. Because placenta was made up of insoluble collagen, the placenta was gelatinized by applying thermal treatment at 90 ℃ for 1 h and used as the sample. The placental hydrolyzing activities of the enzymes at varying concentrations and incubation times were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and gel permeation chromatography (GPC). Based on the SDS-PAGE, the best placental hydrolysis efficiency was observed in trypsin treatments where all peptide bands disappeared after 1 h of incubation as compared to 6 h of chymotrypsin. Pepsin hardly hydrolyzed the placenta as compared to the other two enzymes. The Mw distribution revealed that the trypsin produced placental peptides with Mw of 106 and 500 Da. Peptides produced by chymotrypsin exhibited broad ranges of Mw distribution (1-20 kDa), while the pepsin treatment showed Mw greater than 7 kDa. For comparisons of pre-treatments, the subcritical water processing (37.5 MPa and 200 ℃ of raw placenta improved the efficiency of tryptic digestions to a greater level than that of a preheating treatment (90 ℃ for 1 h). Consequently, subcritical water processing followed by enzymatic digestions has the potential of an advanced collagen hydrolysis technique. |
format | Online Article Text |
id | pubmed-4597853 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Korean Society for Food Science of Animal Resources |
record_format | MEDLINE/PubMed |
spelling | pubmed-45978532016-01-04 Effects of Concentration and Reaction Time of Trypsin, Pepsin, and Chymotrypsin on the Hydrolysis Efficiency of Porcine Placenta Jung, Kyung-Hun Choi, Ye-Chul Chun, Ji-Yeon Min, Sang-Gi Hong, Geun-Pyo Korean J Food Sci Anim Resour Article This study investigated the effects of three proteases (trypsin, pepsin and chymotrypsin) on the hydrolysis efficiency of porcine placenta and the molecular weight (Mw) distributions of the placental hydrolysates. Because placenta was made up of insoluble collagen, the placenta was gelatinized by applying thermal treatment at 90 ℃ for 1 h and used as the sample. The placental hydrolyzing activities of the enzymes at varying concentrations and incubation times were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and gel permeation chromatography (GPC). Based on the SDS-PAGE, the best placental hydrolysis efficiency was observed in trypsin treatments where all peptide bands disappeared after 1 h of incubation as compared to 6 h of chymotrypsin. Pepsin hardly hydrolyzed the placenta as compared to the other two enzymes. The Mw distribution revealed that the trypsin produced placental peptides with Mw of 106 and 500 Da. Peptides produced by chymotrypsin exhibited broad ranges of Mw distribution (1-20 kDa), while the pepsin treatment showed Mw greater than 7 kDa. For comparisons of pre-treatments, the subcritical water processing (37.5 MPa and 200 ℃ of raw placenta improved the efficiency of tryptic digestions to a greater level than that of a preheating treatment (90 ℃ for 1 h). Consequently, subcritical water processing followed by enzymatic digestions has the potential of an advanced collagen hydrolysis technique. Korean Society for Food Science of Animal Resources 2014 2014-04-30 /pmc/articles/PMC4597853/ /pubmed/26760932 http://dx.doi.org/10.5851/kosfa.2014.34.2.151 Text en Copyright © 2014, Korean Society for Food Science of Animal Resources http://creativecommons.org/licences/by-nc/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licences/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Jung, Kyung-Hun Choi, Ye-Chul Chun, Ji-Yeon Min, Sang-Gi Hong, Geun-Pyo Effects of Concentration and Reaction Time of Trypsin, Pepsin, and Chymotrypsin on the Hydrolysis Efficiency of Porcine Placenta |
title | Effects of Concentration and Reaction Time of Trypsin, Pepsin, and Chymotrypsin on the Hydrolysis Efficiency of Porcine Placenta |
title_full | Effects of Concentration and Reaction Time of Trypsin, Pepsin, and Chymotrypsin on the Hydrolysis Efficiency of Porcine Placenta |
title_fullStr | Effects of Concentration and Reaction Time of Trypsin, Pepsin, and Chymotrypsin on the Hydrolysis Efficiency of Porcine Placenta |
title_full_unstemmed | Effects of Concentration and Reaction Time of Trypsin, Pepsin, and Chymotrypsin on the Hydrolysis Efficiency of Porcine Placenta |
title_short | Effects of Concentration and Reaction Time of Trypsin, Pepsin, and Chymotrypsin on the Hydrolysis Efficiency of Porcine Placenta |
title_sort | effects of concentration and reaction time of trypsin, pepsin, and chymotrypsin on the hydrolysis efficiency of porcine placenta |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4597853/ https://www.ncbi.nlm.nih.gov/pubmed/26760932 http://dx.doi.org/10.5851/kosfa.2014.34.2.151 |
work_keys_str_mv | AT jungkyunghun effectsofconcentrationandreactiontimeoftrypsinpepsinandchymotrypsinonthehydrolysisefficiencyofporcineplacenta AT choiyechul effectsofconcentrationandreactiontimeoftrypsinpepsinandchymotrypsinonthehydrolysisefficiencyofporcineplacenta AT chunjiyeon effectsofconcentrationandreactiontimeoftrypsinpepsinandchymotrypsinonthehydrolysisefficiencyofporcineplacenta AT minsanggi effectsofconcentrationandreactiontimeoftrypsinpepsinandchymotrypsinonthehydrolysisefficiencyofporcineplacenta AT honggeunpyo effectsofconcentrationandreactiontimeoftrypsinpepsinandchymotrypsinonthehydrolysisefficiencyofporcineplacenta |