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Activity of select dehydrogenases with Sepharose-immobilized N(6)-carboxymethyl-NAD
N(6)-carboxymethyl-NAD (N(6)-CM-NAD) can be used to immobilize NAD onto a substrate containing terminal primary amines. We previously immobilized N(6)-CM-NAD onto sepharose beads and showed that Thermotoga maritima glycerol dehydrogenase could use the immobilized cofactor with cofactor recycling. We...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4601513/ https://www.ncbi.nlm.nih.gov/pubmed/25611453 http://dx.doi.org/10.1080/21655979.2014.1004020 |