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Evaluating Electroporation and Lipofectamine Approaches for Transient and Stable Transgene Expressions in Human Fibroblasts and Embryonic Stem Cells

OBJECTIVE: Genetic modification of human embryonic stem cells (hESCs) is critical for their extensive use as a fundamental tool for cell therapy and basic research. Despite the fact that various methods such as lipofection and electroporation have been applied to transfer the gene of interest (GOI)...

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Detalles Bibliográficos
Autores principales: Sharifi Tabar, Mehdi, Hesaraki, Mahdi, Esfandiari, Fereshteh, Sahraneshin Samani, Fazel, Vakilian, Haghighat, Baharvand, Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4601864/
https://www.ncbi.nlm.nih.gov/pubmed/26464815