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Evaluating Electroporation and Lipofectamine Approaches for Transient and Stable Transgene Expressions in Human Fibroblasts and Embryonic Stem Cells
OBJECTIVE: Genetic modification of human embryonic stem cells (hESCs) is critical for their extensive use as a fundamental tool for cell therapy and basic research. Despite the fact that various methods such as lipofection and electroporation have been applied to transfer the gene of interest (GOI)...
Autores principales: | Sharifi Tabar, Mehdi, Hesaraki, Mahdi, Esfandiari, Fereshteh, Sahraneshin Samani, Fazel, Vakilian, Haghighat, Baharvand, Hossein |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4601864/ https://www.ncbi.nlm.nih.gov/pubmed/26464815 |
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