Characterization of plasma protein binding dissociation with online SPE-HPLC

A novel parameter of relative recovery (R(re)) was defined and determined by online SPE-HPLC to characterize plasma protein binding (PPB) kinetics of highly plasma binding drugs. The proportional relationship of R(re) with k(off) of PPB has been established with a new SPE model. A rapid, easy to use...

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Detalles Bibliográficos
Autores principales: Li, Ping, Fan, Yiran, Wang, Yunlong, Lu, Yaxin, Yin, Zheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4602217/
https://www.ncbi.nlm.nih.gov/pubmed/26460813
http://dx.doi.org/10.1038/srep14866
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author Li, Ping
Fan, Yiran
Wang, Yunlong
Lu, Yaxin
Yin, Zheng
author_facet Li, Ping
Fan, Yiran
Wang, Yunlong
Lu, Yaxin
Yin, Zheng
author_sort Li, Ping
collection PubMed
description A novel parameter of relative recovery (R(re)) was defined and determined by online SPE-HPLC to characterize plasma protein binding (PPB) kinetics of highly plasma binding drugs. The proportional relationship of R(re) with k(off) of PPB has been established with a new SPE model. A rapid, easy to use method could potentially be used to categorize PK properties of the drug candidates in the decision process of drug discovery and development.
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spelling pubmed-46022172015-10-23 Characterization of plasma protein binding dissociation with online SPE-HPLC Li, Ping Fan, Yiran Wang, Yunlong Lu, Yaxin Yin, Zheng Sci Rep Article A novel parameter of relative recovery (R(re)) was defined and determined by online SPE-HPLC to characterize plasma protein binding (PPB) kinetics of highly plasma binding drugs. The proportional relationship of R(re) with k(off) of PPB has been established with a new SPE model. A rapid, easy to use method could potentially be used to categorize PK properties of the drug candidates in the decision process of drug discovery and development. Nature Publishing Group 2015-10-13 /pmc/articles/PMC4602217/ /pubmed/26460813 http://dx.doi.org/10.1038/srep14866 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Li, Ping
Fan, Yiran
Wang, Yunlong
Lu, Yaxin
Yin, Zheng
Characterization of plasma protein binding dissociation with online SPE-HPLC
title Characterization of plasma protein binding dissociation with online SPE-HPLC
title_full Characterization of plasma protein binding dissociation with online SPE-HPLC
title_fullStr Characterization of plasma protein binding dissociation with online SPE-HPLC
title_full_unstemmed Characterization of plasma protein binding dissociation with online SPE-HPLC
title_short Characterization of plasma protein binding dissociation with online SPE-HPLC
title_sort characterization of plasma protein binding dissociation with online spe-hplc
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4602217/
https://www.ncbi.nlm.nih.gov/pubmed/26460813
http://dx.doi.org/10.1038/srep14866
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