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An improved method for detecting circulating microRNAs with S-Poly(T) Plus real-time PCR

We herein describe a simple, sensitive and specific method for analysis of circulating microRNAs (miRNA), termed S-Poly(T) Plus real-time PCR assay. This new method is based on our previously developed S-Poly(T) method, in which a unique S-Poly(T) primer is used during reverse-transcription to incre...

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Detalles Bibliográficos
Autores principales: Niu, Yanqin, Zhang, Limin, Qiu, Huiling, Wu, Yike, Wang, Zhiwei, Zai, Yujia, Liu, Lin, Qu, Junle, Kang, Kang, Gou, Deming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4602224/
https://www.ncbi.nlm.nih.gov/pubmed/26459910
http://dx.doi.org/10.1038/srep15100