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An improved method for detecting circulating microRNAs with S-Poly(T) Plus real-time PCR
We herein describe a simple, sensitive and specific method for analysis of circulating microRNAs (miRNA), termed S-Poly(T) Plus real-time PCR assay. This new method is based on our previously developed S-Poly(T) method, in which a unique S-Poly(T) primer is used during reverse-transcription to incre...
Autores principales: | Niu, Yanqin, Zhang, Limin, Qiu, Huiling, Wu, Yike, Wang, Zhiwei, Zai, Yujia, Liu, Lin, Qu, Junle, Kang, Kang, Gou, Deming |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4602224/ https://www.ncbi.nlm.nih.gov/pubmed/26459910 http://dx.doi.org/10.1038/srep15100 |
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