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Patterned polymer matrix promotes stemness and cell-cell interaction of adult stem cells
BACKGROUND: The interaction of stem cells with their culture substrates is critical in controlling their fate and function. Declining stemness of adult-derived human mesenchymal stem cells (hMSCs) during in vitro expansion on tissue culture polystyrene (TCPS) severely limits their therapeutic effica...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4603908/ https://www.ncbi.nlm.nih.gov/pubmed/26464581 http://dx.doi.org/10.1186/s13036-015-0016-x |
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author | Hofmeister, Lucas H. Costa, Lino Balikov, Daniel A. Crowder, Spencer W. Terekhov, Alexander Sung, Hak-Joon Hofmeister, William H. |
author_facet | Hofmeister, Lucas H. Costa, Lino Balikov, Daniel A. Crowder, Spencer W. Terekhov, Alexander Sung, Hak-Joon Hofmeister, William H. |
author_sort | Hofmeister, Lucas H. |
collection | PubMed |
description | BACKGROUND: The interaction of stem cells with their culture substrates is critical in controlling their fate and function. Declining stemness of adult-derived human mesenchymal stem cells (hMSCs) during in vitro expansion on tissue culture polystyrene (TCPS) severely limits their therapeutic efficacy prior to cell transplantation into damaged tissues. Thus, various formats of natural and synthetic materials have been manipulated in attempts to reproduce in vivo matrix environments in which hMSCs reside. RESULTS: We developed a series of patterned polymer matrices for cell culture by hot-pressing poly(ε-caprolactone) (PCL) films in femtosecond laser-ablated nanopore molds, forming nanofibers on flat PCL substrates. hMSCs cultured on these PCL fiber matrices significantly increased expression of critical self-renewal factors, Nanog and OCT4A, as well as markers of cell-cell interaction PECAM and ITGA2. The results suggest the patterned polymer fiber matrix is a promising model to maintain the stemness of adult hMSCs. CONCLUSION: This approach meets the need for scalable, highly repeatable, and tuneable models that mimic extracellular matrix features that signal for maintenance of hMSC stemness. |
format | Online Article Text |
id | pubmed-4603908 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46039082015-10-14 Patterned polymer matrix promotes stemness and cell-cell interaction of adult stem cells Hofmeister, Lucas H. Costa, Lino Balikov, Daniel A. Crowder, Spencer W. Terekhov, Alexander Sung, Hak-Joon Hofmeister, William H. J Biol Eng Research BACKGROUND: The interaction of stem cells with their culture substrates is critical in controlling their fate and function. Declining stemness of adult-derived human mesenchymal stem cells (hMSCs) during in vitro expansion on tissue culture polystyrene (TCPS) severely limits their therapeutic efficacy prior to cell transplantation into damaged tissues. Thus, various formats of natural and synthetic materials have been manipulated in attempts to reproduce in vivo matrix environments in which hMSCs reside. RESULTS: We developed a series of patterned polymer matrices for cell culture by hot-pressing poly(ε-caprolactone) (PCL) films in femtosecond laser-ablated nanopore molds, forming nanofibers on flat PCL substrates. hMSCs cultured on these PCL fiber matrices significantly increased expression of critical self-renewal factors, Nanog and OCT4A, as well as markers of cell-cell interaction PECAM and ITGA2. The results suggest the patterned polymer fiber matrix is a promising model to maintain the stemness of adult hMSCs. CONCLUSION: This approach meets the need for scalable, highly repeatable, and tuneable models that mimic extracellular matrix features that signal for maintenance of hMSC stemness. BioMed Central 2015-10-12 /pmc/articles/PMC4603908/ /pubmed/26464581 http://dx.doi.org/10.1186/s13036-015-0016-x Text en © Hofmeister et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Hofmeister, Lucas H. Costa, Lino Balikov, Daniel A. Crowder, Spencer W. Terekhov, Alexander Sung, Hak-Joon Hofmeister, William H. Patterned polymer matrix promotes stemness and cell-cell interaction of adult stem cells |
title | Patterned polymer matrix promotes stemness and cell-cell interaction of adult stem cells |
title_full | Patterned polymer matrix promotes stemness and cell-cell interaction of adult stem cells |
title_fullStr | Patterned polymer matrix promotes stemness and cell-cell interaction of adult stem cells |
title_full_unstemmed | Patterned polymer matrix promotes stemness and cell-cell interaction of adult stem cells |
title_short | Patterned polymer matrix promotes stemness and cell-cell interaction of adult stem cells |
title_sort | patterned polymer matrix promotes stemness and cell-cell interaction of adult stem cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4603908/ https://www.ncbi.nlm.nih.gov/pubmed/26464581 http://dx.doi.org/10.1186/s13036-015-0016-x |
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