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Single cell PCR amplification of diatoms using fresh and preserved samples

Single cell Chelex® DNA extraction and nested PCR amplification were used to examine partial gene sequences from natural diatom populations for taxonomic and phylogenetic studies at and above the level of species. DNA was extracted from cells that were either fresh collected or stored in RNAlater. E...

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Detalles Bibliográficos
Autores principales: Hamilton, Paul B., Lefebvre, Keely E., Bull, Roger D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4604310/
https://www.ncbi.nlm.nih.gov/pubmed/26528252
http://dx.doi.org/10.3389/fmicb.2015.01084