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Determining the N-terminal orientations of recombinant transmembrane proteins in the Escherichia coli plasma membrane

In silico algorithms have been the common approach for transmembrane (TM) protein topology prediction. However, computational tools may produce questionable results and experimental validation has proven difficult. Although biochemical strategies are available to determine the C-terminal orientation...

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Detalles Bibliográficos
Autores principales: Lee, Chien-Hsien, Chou, Chia-Cheng, Hsu, Min-Feng, Wang, Andrew H.-J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4604451/
https://www.ncbi.nlm.nih.gov/pubmed/26462555
http://dx.doi.org/10.1038/srep15086