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Determining the N-terminal orientations of recombinant transmembrane proteins in the Escherichia coli plasma membrane
In silico algorithms have been the common approach for transmembrane (TM) protein topology prediction. However, computational tools may produce questionable results and experimental validation has proven difficult. Although biochemical strategies are available to determine the C-terminal orientation...
Autores principales: | Lee, Chien-Hsien, Chou, Chia-Cheng, Hsu, Min-Feng, Wang, Andrew H.-J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4604451/ https://www.ncbi.nlm.nih.gov/pubmed/26462555 http://dx.doi.org/10.1038/srep15086 |
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