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Three-dimensional super-resolution protein localization correlated with vitrified cellular context

We demonstrate the use of cryogenic super-resolution correlative light and electron microscopy (csCLEM) to precisely determine the spatial relationship between proteins and their native cellular structures. Several fluorescent proteins (FPs) were found to be photoswitchable and emitted far more phot...

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Detalles Bibliográficos
Autores principales: Liu, Bei, Xue, Yanhong, Zhao, Wei, Chen, Yan, Fan, Chunyan, Gu, Lusheng, Zhang, Yongdeng, Zhang, Xiang, Sun, Lei, Huang, Xiaojun, Ding, Wei, Sun, Fei, Ji, Wei, Xu, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4604464/
https://www.ncbi.nlm.nih.gov/pubmed/26462878
http://dx.doi.org/10.1038/srep13017