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The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli
Genome engineering methods in E. coli allow for easy to perform manipulations of the chromosome in vivo with the assistance of the λ-Red recombinase system. These methods generally rely on the insertion of an antibiotic resistance cassette followed by removal of the same cassette, resulting in a two...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group
2015
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4604488/ https://www.ncbi.nlm.nih.gov/pubmed/26463009 http://dx.doi.org/10.1038/srep15096 |